Project

Part:BBa_K787002:Design

Designed by: Anish Vaghela   Group: iGEM12_Rutgers   (2012-10-02)


pCWORi-ter-adhE2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1406
    Illegal PstI site found at 370
    Illegal PstI site found at 5307
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1406
    Illegal PstI site found at 370
    Illegal PstI site found at 5307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1406
    Illegal BglII site found at 4044
    Illegal BglII site found at 4080
    Illegal BamHI site found at 186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1406
    Illegal PstI site found at 370
    Illegal PstI site found at 5307
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1406
    Illegal PstI site found at 370
    Illegal PstI site found at 5307
    Illegal NgoMIV site found at 4306
    Illegal AgeI site found at 1998
    Illegal AgeI site found at 3267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5481
    Illegal SapI.rc site found at 6563


Design Notes

Many illegal sites.


Source

Source: "Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways"

http://www.ncbi.nlm.nih.gov/pubmed/21358636

References